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sequence-specific fluorescent oligonucleotide taqman probes and primers  (Thermo Fisher)


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    Thermo Fisher sequence-specific fluorescent oligonucleotide taqman probes and primers
    Sequence Specific Fluorescent Oligonucleotide Taqman Probes And Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sequence-specific+oligonucleotide+probes/sequence+specific+fluorescent+oligonucleotide+taqman+probes+and+primers/pmc11790506-356-7-8
    Average 90 stars, based on 1 article reviews
    sequence-specific fluorescent oligonucleotide taqman probes and primers - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: A fast and reliable method for detecting SNP rs67384697 (Hsa-miR-148a binding site) by a single run of allele-specific real-time PCR.
    Article Snippet: Unit of Human Virology, Division of Immunology, Transplantation and Infectious Diseases IRCCS Ospedale San Raffaele, Milan, Italy Translational Research Department, Laboratory Medicine, Diagnostics and Services, IRCCS Istituto Giannina Gaslini, Genoa, Italy Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy Histocompatibility Laboratory, Galliera Hospital, Genoa, Italy

    Article Title: KIR3DS1-Mediated Recognition of HLA-*B51: Modulation of KIR3DS1 Responsiveness by Self HLA-B Allotypes and Effect on NK Cell Licensing
    Article Snippet: Exons 2, 3, and 4 were bidirectionally sequenced using an ABI 3130xl Genetic Analyzer (Applied Biosystems, Foster City, CA, USA), and the sequences were analyzed by Assign 3.5+ HARPS software (Conexio Genomics, Applecross, Australia). .. Some HLA typings were performed using sequence-specific oligonucleotide probes (PCR-SSOP; One Lambda, Canoga Park, CA, USA), and the results were analyzed by the software Fusion 3.0 (One Lambda, Canoga Park, CA, USA). ..

    Article Title: Identification of immunodominant HLA-B7-restricted CD8 + cytotoxic T cell epitopes derived from mammaglobin-A expressed on human breast cancers
    Article Snippet: .. HLA typing was performed using sequence-specific oligonucleotide probes that provide low-medium resolution for HLA-B alleles (Dynal Biotech, Lafayette Hill, PA). .. Breast cancer cell lines were obtained from the American Type Culture Collection (Manassas, VA) ( ).

    Article Title: C1q-binding DSA and allograft outcomes in pediatric kidney transplant recipients.
    Article Snippet: In children with advanced CKD or end-stage renal disease (ESRD), renal transplantation is associated with improved outcomes when compared to children on dialysis.1,2 While short-term outcomes have continued to improve with advanced surgical techniques and newer immunosuppressive agents,3 the development of antibody-mediated (ABMR) is a barrier to the long-term survival of the kidney allograft.4,5 The presence of donor-specific antibody (DSA) is a well-known independent risk factor for the development of Received: 26 June 2020 | Revised: 11 September 2020 | Accepted: 25 September 2020 DOI: 10.1111/petr.13885

    Article Title: Single Nucleotide Transcription Factor 7-Like 2 ( TCF7L2 ) Gene Polymorphisms in Antiislet Autoantibody-Negative Patients at Onset of Diabetes
    Article Snippet: .. HLA class II subtyping was performed by PCR amplification of the polymorphic exon 2 of the HLA - DQA1-DQB1 gene and hybridization of amplified DNA using sequence-specific oligonucleotide probes (Applied Biosystems; and Dynal Biotech, Oslo, Norway). .. HLA-DRB1 was subtyped by sequencing of the PCR-amplified exon 2 with alleles called by Matchmaker (Celera Genomics, Rockville, MD).

    Article Title: Donor-specific HLA antibodies in predicting crossmatch outcome: Comparison of three different laboratory techniques.
    Article Snippet: 3.2 Donor HLA typing Donors were HLA typed with the complement-mediated lymphocytotoxicity test (Biotest Rockaway, NJ) and a low-resolution polymerase chain reaction AC C EP TE D M AN U SC R IP T with sequence-specific primers (One Lambda Inc., Canoga Park, CA). .. Supplementary typing for HLA-A, B, C, DRB1-5, DQA1, DQB1, DPA1 and DPB1 was performed with a polymerase chain reaction with sequence-specific oligonucleotide probes (One Lambda) when the result was needed to confirm the donor-specificity of an antibody. ..

    Article Title: Combined Analysis of HLA Class II Eplet Mismatch and Tacrolimus Levels for the Prediction of De Novo Donor Specific Antibody Development in Kidney Transplant Recipients
    Article Snippet: .. High-resolution HLA typing for DRB1, DQB1, and DQA1 was performed using sequence-specific oligonucleotide probes (One Lambda Inc., A Thermo Fisher Scientific Brand, Canoga Park, CA, USA). ..

    Software:

    Article Title: A fast and reliable method for detecting SNP rs67384697 (Hsa-miR-148a binding site) by a single run of allele-specific real-time PCR.
    Article Snippet: Unit of Human Virology, Division of Immunology, Transplantation and Infectious Diseases IRCCS Ospedale San Raffaele, Milan, Italy Translational Research Department, Laboratory Medicine, Diagnostics and Services, IRCCS Istituto Giannina Gaslini, Genoa, Italy Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy Histocompatibility Laboratory, Galliera Hospital, Genoa, Italy

    Article Title: KIR3DS1-Mediated Recognition of HLA-*B51: Modulation of KIR3DS1 Responsiveness by Self HLA-B Allotypes and Effect on NK Cell Licensing
    Article Snippet: Exons 2, 3, and 4 were bidirectionally sequenced using an ABI 3130xl Genetic Analyzer (Applied Biosystems, Foster City, CA, USA), and the sequences were analyzed by Assign 3.5+ HARPS software (Conexio Genomics, Applecross, Australia). .. Some HLA typings were performed using sequence-specific oligonucleotide probes (PCR-SSOP; One Lambda, Canoga Park, CA, USA), and the results were analyzed by the software Fusion 3.0 (One Lambda, Canoga Park, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: KIR3DS1-Mediated Recognition of HLA-*B51: Modulation of KIR3DS1 Responsiveness by Self HLA-B Allotypes and Effect on NK Cell Licensing
    Article Snippet: Exons 2, 3, and 4 were bidirectionally sequenced using an ABI 3130xl Genetic Analyzer (Applied Biosystems, Foster City, CA, USA), and the sequences were analyzed by Assign 3.5+ HARPS software (Conexio Genomics, Applecross, Australia). .. Some HLA typings were performed using sequence-specific oligonucleotide probes (PCR-SSOP; One Lambda, Canoga Park, CA, USA), and the results were analyzed by the software Fusion 3.0 (One Lambda, Canoga Park, CA, USA). ..

    Article Title: Single Nucleotide Transcription Factor 7-Like 2 ( TCF7L2 ) Gene Polymorphisms in Antiislet Autoantibody-Negative Patients at Onset of Diabetes
    Article Snippet: .. HLA class II subtyping was performed by PCR amplification of the polymorphic exon 2 of the HLA - DQA1-DQB1 gene and hybridization of amplified DNA using sequence-specific oligonucleotide probes (Applied Biosystems; and Dynal Biotech, Oslo, Norway). .. HLA-DRB1 was subtyped by sequencing of the PCR-amplified exon 2 with alleles called by Matchmaker (Celera Genomics, Rockville, MD).

    Article Title: Donor-specific HLA antibodies in predicting crossmatch outcome: Comparison of three different laboratory techniques.
    Article Snippet: 3.2 Donor HLA typing Donors were HLA typed with the complement-mediated lymphocytotoxicity test (Biotest Rockaway, NJ) and a low-resolution polymerase chain reaction AC C EP TE D M AN U SC R IP T with sequence-specific primers (One Lambda Inc., Canoga Park, CA). .. Supplementary typing for HLA-A, B, C, DRB1-5, DQA1, DQB1, DPA1 and DPB1 was performed with a polymerase chain reaction with sequence-specific oligonucleotide probes (One Lambda) when the result was needed to confirm the donor-specificity of an antibody. ..

    Immunopeptidomics:

    Article Title: Identification of immunodominant HLA-B7-restricted CD8 + cytotoxic T cell epitopes derived from mammaglobin-A expressed on human breast cancers
    Article Snippet: .. HLA typing was performed using sequence-specific oligonucleotide probes that provide low-medium resolution for HLA-B alleles (Dynal Biotech, Lafayette Hill, PA). .. Breast cancer cell lines were obtained from the American Type Culture Collection (Manassas, VA) ( ).

    Article Title: Combined Analysis of HLA Class II Eplet Mismatch and Tacrolimus Levels for the Prediction of De Novo Donor Specific Antibody Development in Kidney Transplant Recipients
    Article Snippet: .. High-resolution HLA typing for DRB1, DQB1, and DQA1 was performed using sequence-specific oligonucleotide probes (One Lambda Inc., A Thermo Fisher Scientific Brand, Canoga Park, CA, USA). ..

    other:

    Article Title: Identification of immunodominant HLA-B7-restricted CD8 + cytotoxic T cell epitopes derived from mammaglobin-A expressed on human breast cancers
    Article Snippet: MGBA expression was determined by reverse transcriptase–polymerase chain reaction and western blotting as previously described [ 15 , 19 ]. table ft1 table-wrap mode="anchored" t5 caption a7 Breast cancer cell line Tumor classification HLA-B genotype a MGBA expression (mRNA) b MGBA expression (protein) c DU-4475 Adenocarcinoma HLA-B7,40 + + AU-565 Adenocarcinoma HLA-B14,40 + + MDA-361 Adenocarcinoma HLA-B39,51 + + MDA-231 Adenocarcinoma HLA-B40,41 − − Open in a separate window a HLA typing was performed using sequence-specific oligonueleotide probes that provided low-medium resolution for HLA-B alleles (Dynal Biotech) b Determined by reverse transcriptase–polymerase chain reaction as previously described [ 17 ] c Determined by western blotting as previously described [ 17 ] Breast cancer cell lines

    Amplification:

    Article Title: Single Nucleotide Transcription Factor 7-Like 2 ( TCF7L2 ) Gene Polymorphisms in Antiislet Autoantibody-Negative Patients at Onset of Diabetes
    Article Snippet: .. HLA class II subtyping was performed by PCR amplification of the polymorphic exon 2 of the HLA - DQA1-DQB1 gene and hybridization of amplified DNA using sequence-specific oligonucleotide probes (Applied Biosystems; and Dynal Biotech, Oslo, Norway). .. HLA-DRB1 was subtyped by sequencing of the PCR-amplified exon 2 with alleles called by Matchmaker (Celera Genomics, Rockville, MD).

    Hybridization:

    Article Title: Single Nucleotide Transcription Factor 7-Like 2 ( TCF7L2 ) Gene Polymorphisms in Antiislet Autoantibody-Negative Patients at Onset of Diabetes
    Article Snippet: .. HLA class II subtyping was performed by PCR amplification of the polymorphic exon 2 of the HLA - DQA1-DQB1 gene and hybridization of amplified DNA using sequence-specific oligonucleotide probes (Applied Biosystems; and Dynal Biotech, Oslo, Norway). .. HLA-DRB1 was subtyped by sequencing of the PCR-amplified exon 2 with alleles called by Matchmaker (Celera Genomics, Rockville, MD).



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